Laser radiation. Documentation of admissible values of the intensity of the physical factor Agnieszka Wolska, Piotr Głogowski   Methods of evaluation of moderate thermal environment according to EN ISO 7730 Anna Bogdan    4-Aminobiphenyl – determination method Anna Jeżewska, Bogusław Buszewski n-Butane – determination method   Anna Jeżewska 1,3-Dichloropropanol-2 – determination method I   Barbara Romanowicz, Jan Gromiec 1,3-Dichloropropanol-2 – determination method II Barbara Romanowicz, Jan Gromiec  4,4’-Methylenebis(2-chloroaniline) – determination method Anna Jeżewska, Bogusław Buszewski Diphrophyllinum – determination method Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz Chloromethyl methyl ether – determination method Barbara Romanowicz, Jan Gromiec 2-Ethoxyethanole – determination method   Barbara Romanowicz, Jan Gromiec N-Hydroxyurea – determination method   Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz Cobalt and its compounds – determination method   Ewa Gawęda  2-Methoxyethanole – determination method Barbara Romanowicz, Jan Gromiec   2-Ethoxyethyl acetate – determination method   Wiktor Wesołowski, Małgorzata Kucharska, Jan Gromiec   2-Methoxyethyl acetate – determination method Małgorzata Kucharska, Wiktor Wesołowski, Jan Gromiec   Tetraethyl silicate – determination method   Joanna Kowalska    Propane – determination method Agnieszka Woźnica   Sulphyryde – determination method   Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz   Thioacetamide – determination method   Wiktor Wesołowski, Małgorzata Kucharska Magnesium oxide – determination method   Ewa Gawęda   Hydrogenated terphenyls – determination method   Wiktor Wesołowski, Małgorzata Kucharska, Jan Gromiec Potassium hydroxide – determination method   Jolanta Surgiewicz

Laser radiation. Documentation of admissible values of the intensity of the physical factor  
Agnieszka Wolska, Piotr Głogowski  
Directive 2006/25/EC lists exposure limit values, which are derived from current scientific studies on the influence of laser radiation on biological tissues. Because of the obligation to transpose that directive into Polish legislation it was necessary to compare currently obligatory maximum permissible exposures (MPEs) with the exposure limit values in directive. As differences were found between those values, it was necessary to develop suitable documentation of maximum admissible intensities of laser radiation and to submit it to the Interdepartmental Commission for Maximum Admissible Concentrations and Intensities for Agents Harmful to Health in the Working Environment. The documentation contains an analysis of potential hazards related to the influence of laser radiation on biological tissues, statistics on accidents related to laser radiation, a comparison of MPEs in the Ordinance of Ministry of Economy, Labour and Social Affairs of 29th November 2002 on maximum admissible concentrations and intensities for agents harmful to health in the working environment with the exposure limit values in directive 2006/25/EC, a proposal for new legislation in p. 4 “Laser radiation” in part D of Attachment 2 to the above-mentioned ordinance (new MPEs), grounds for changing the introduction in that ordinance, and the scope of preliminary and periodic medical examinations of workers exposed to laser radiation.

 Methods of evaluation of moderate thermal environment according to EN ISO 7730
Anna Bogdan  
Provide employees comfort sensation by adjusting the particular parameters of work environment translates into reduce errors, reduce the number of work accidents and occupational diseases, as well as to improve productivity and quality of products and services. According to data gathered in 2008 about 60 thousand people in Poland are employed in conditions of cold and hot environment, which means that the majority of population is exposed to moderate environment, and hence in the environment, which should meet the requirements of thermal comfort, while the users often perceived as unsatisfying.
The paper presents methods for assessing general and local thermal comfort of employees.

 4-Aminobiphenyl – determination method
Anna Jeżewska, Bogusław Buszewski  
The method is based on the chemisorption of 4-aminobiphenyl on a glass fiber filter treated with sulphuric acid, followed by extraction of 4-aminobiphenyl sulphate with water  and  NaOH  solution. 4-Aminobiphenyl is eluted from an SPE cartridge using 1 mL of methanol. The obtained solution is analyzed with HPLC with FL detection.
The working range is 0.1–2 μg/m³ for a 100-L air sample.

n-Butane – determination method  
Anna Jeżewska
Determination of a worker’s exposure to airborne n-butane is made by using an Anasorb CMS tube (400/200 mg sections). Samples are collected at a maximum flow rate of 1.5 L/h until a maximum collection volume of 3 L is reached. After sampling, the Anasorb CMS tube is desorbed in n-hexane. The obtained solution is analyzed with gas chromatography (GC-FID).
The working range is 190 ÷ 3800 mg/m³ for a 3-L air sample.

1,3-Dichloropropanol-2 – determination method I  
Barbara Romanowicz, Jan Gromiec
The method is based on the adsorption of 1,3-dichloropropanol-2 vapours on Tenax. Samples are desorbed with acetone and analyzed by gas chromatography with a flame ionization detector (GC-FID).
The determination limit of the method is 0.5 mg/m³  for  10 l air sample.

1,3-Dichloropropanol-2 – determination method II  
Barbara Romanowicz, Jan Gromiec  
The method is based on the adsorption of 1,3-dichloropropanol-2 vapours on Tenax. Samples are desorbed with acetone and analyzed by gas chromatography with an electron capture detector (GC-ECD).  
The determination limit of the method is  0.005 mg/m³.

4,4’-Methylenebis(2-chloroaniline) – determination method
Anna Jeżewska, Bogusław Buszewski  
The method is based on the chemisorption of 4,4’-methylenebis(2-chloroaniline) on the glass fiber filter treated with sulphuric acid, followed by extraction of amine with water. Quantitation is performed by analyzing the 3,5-dinitrobenzoyl chloride derivatives of the 4,4’-methylenebis(2-chloroaniline) by HPLC with DAD detection.
The working range is 2 to 40 μg/m³ for a 100-L air sample.

Diphrophyllinum – determination method  
Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz
This method is based on deposition of diphrophyllinum on glass fiber filters, desorption with distillated water and chromatographic (HPLC) analysis of the resulting solution.
The determination limit of this method is 0.01 mg/m³.

Chloromethyl methyl ether – determination method  
Barbara Romanowicz, Jan Gromiec
Chloromethyl methyl ether (CMME) is collected in a reactant-coated solid adsorbent tube forming a trichlorophenol derivative. The collected CMME derivative is desorbed with methanol, cleaned up with aqueous KOH, extracted into hexane and analyzed with gas chromatography with an electron capture detector (GC-ECD). The determination limit of this method is  0.02 mg/m³.

 2-Ethoxyethanole – determination method  
Barbara Romanowicz, Jan Gromiec
This method is based on the adsorption of  2-ethoxyethanole vapours on activated charcoal. Samples are desorbed with 95/5 (v/v) methylene chloride/methanol and analysed with gas chromatography using flame ionization detection (GC-FID).
The determination limit of the method is  0.8 mg/m³  for  a 5-L air sample.

 N-Hydroxyurea – determination method  
Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz
This method is based on deposition of N-hydroxyurea on glass fiber filters, desorption with distillated water and chromatographic (HPLC) analysis of the resulting solution.
The determination limit of this method is 0.01 mg/m³.

 Cobalt and its compounds – determination method  
Ewa Gawęda
This method is based on stopping cobalt and its compounds on a membrane filter, dissoling the sample in aqua-regia and then mineralizing it with aqua-regia and concentrated nitric acid and preparating the solution for analysis. Cobalt in the solution is determined with atomic absorption spectrophotometry with a graphite tube.
The determination limit of this method is 0.002 mg/m³.

2-Methoxyethanole – determination method  
Barbara Romanowicz, Jan Gromiec  
This method is based on the adsorption of 2-methoxyethanole vapours on activated charcoal. Samples are desorbed with 5/95 (v/v) methanol/methylene chloride and analysed with gas chromatography using flame ionization detection (GC-FID).
The determination limit of this method is  0.3 mg/m³.

 2-Ethoxyethyl acetate – determination method  
Wiktor Wesołowski, Małgorzata Kucharska, Jan Gromiec  
The method is based on the adsorption of 2-ethoxyethyl acetate on activated charcoal, desorption with dichloromethane and gas chromatographic (GC-MSD) analysis of the resulting solution.
The determination limit of the method is 0.1 mg/m³.

2-Methoxyethyl acetate – determination method  
Małgorzata Kucharska, Wiktor Wesołowski, Jan Gromiec  
The method is based on the adsorption of 2-methoxyethyl acetate on activated charcoal, desorption with dichloromethane and gas chromatographic (GC-MSD) analysis of the resulting solution.
The determination limit of the method is 0.05 mg/m³.

Tetraethyl silicate – determination method    
Joanna Kowalska  
This determination method is based on the adsorption of tetraethyl silicate vapours on XAD-2 resin (100/50 mg sections), desorption with acetonitryle and gas chromatography with flame ionization detection (GC-FID) analysis of the resulting solution.
The determination limit of this method is 7.82 mg/m³.

Propane – determination method  
Agnieszka Woźnica  
Workers’ exposure to airborne propane is determined with an Anasorb CMS tube (400/200 mg sections). Samples are collected at a maximum flow rate of 1.5 L/h until a maximum collection volume of 3 L is reached. After sampling, the Anasorb CMS tube is desorbed in n-hexane. The obtained solution is analyzed with gas chromatography (GC-FID).
The working range for a 3-L air sample is 180–3600 mg/m³.

 Sulphyryde – determination method  
Małgorzata Szewczyńska, Anna Osytek, Małgorzata Pośniak, Magdalena Galwas-Grzeszkiewicz  
This method is based on deposition of sulphyryde on glass fiber filters, desorption with distillated water and chromatographic (HPLC) analysis of the resulting solution.
The determination limit of this method is 0.01 mg/m³.

 Thioacetamide – determination method  
Wiktor Wesołowski, Małgorzata Kucharska
Air samples are collected by drawing a know volume of air through glass microfibre filters. Samples are extracted with acetone by means of an ultrasonic bath. The obtained extracts are analyzed with gas chromatography with mass spectrometry (GC-MS).
The determination limit of the method is 0.01 mg/m³.

Magnesium oxide – determination method    
Ewa Gawęda
This method is based on stopping magnesium oxide on a membrane filter, mineralizing the sample with concentrated nitric acid and preparing the solution for analysis. Magnesium oxide in the solution is determined with flame atomic absorption spectrophotometry, as magnesium.
The determination limit of this method is 0.46 mg/m³.

 Hydrogenated terphenyls – determination method  
Wiktor Wesołowski, Małgorzata Kucharska, Jan Gromiec
Air samples are collected by drawing a known volume of air through glass fibre filters  in series with  XAD-2 tubes. Samples are extracted in an ultrasonic bath with 2 ml dichloromethane. The obtained extracts are analyzed with gas chromatography using a mass detector (GC-MSD).
The determination limit of this method is 1 mg/m³ for hydrogenated terphenyls and 0.0001 mg/m³ for o- terphenyl, 0.0005 mg/m³ for m-terphenyl, 0.0004 mg/m3 for p-terphenyl.

Potassium hydroxide – determination method    
Jolanta Surgiewicz  
This method is based on stopping selected potassium hydroxide on a membrane filter, mineralizing the sample with concentration nitric acid and preparing the solution for analysis in diluted nitric acid and caesium chloride. Potassium hydroxide in the solution is determined as potassium with flame atomic absorption spectrometry.
The detection limit of determined potassium hydroxide for this method is 0.04 mg/m³.